22 Publications
2025
A Role for Two Conserved Arginine Residues in Protected Persulfide Transfer by SufE-Dependent SufS Cysteine Desulfurases. Biochemistry
. | Journal Article
 
Cas10 residues lining the target RNA binding channel regulate interference by distinguishing cognate target RNA from mismatched targets
 
Two dynamic N-terminal regions are required for function in ribosomal RNA adenine dimethylase family members. RNA
. | Journal Article
2024
The structure of the SufS-SufE complex reveals interactions driving protected persulfide transfer in iron-sulfur cluster biogenesis
 
The structure of the SufS–SufE complex reveals interactions driving protected persulfide transfer in iron-sulfur cluster biogenesis. Journal of Biological Chemistry
. | Journal Article
 
Two conserved arginine residues facilitate C-S bond cleavage and persulfide transfer in Suf family cysteine desulfurases
 
Two dynamic, N-terminal regions are required for function in Ribosomal RNA Adenine Dimethylase family members
2023
The structure of a Type III-A CRISPR-Cas effector complex reveals conserved and idiosyncratic contacts to target RNA and crRNA among Type III-A systems. PLOS ONE
. | Journal Article
 
The β-latch structural element of the SufS cysteine desulfurase mediates active site accessibility and SufE transpersulfurase positioning
Gogar, Rajleen K⋅Carroll, Franki⋅Conte, Juliana V⋅Nasef, Mohamed⋅Dunkle, Jack A and Frantom, Patrick A
The Journal of biological chemistry, pp. 102966, Jan 31, 2023. | Journal Article
2022
The effect of crRNA–target mismatches on cOA-mediated interference by a type III-A CRISPR-Cas system. RNA Biology
. | Journal Article
 
The structure of a Type III-A CRISPR-Cas effector complex reveals conserved and idiosyncratic contacts to target RNA and crRNA among Type III-A systems
 
Three critical regions of the erythromycin resistance methyltransferase, ErmE, are required for function supporting a model for the interaction of Erm family enzymes with substrate rRNA
Sharkey, Rory E.⋅Herbert, Johnny B.⋅McGaha, Danielle A.⋅Nguyen, Vy⋅Schoeffler, Allyn J. and Dunkle, Jack A.
RNA, vol. 28, pp. 210–226. | Journal Article
2020
Shared requirements for key residues in the antibiotic resistance enzymes ErmC and ErmE suggest a common mode of RNA recognition
Rowe, Sebastian J.⋅Mecaskey, Ryan J.⋅Nasef, Mohamed⋅Talton, Rachel C.⋅Sharkey, Rory E.⋅Halliday, Joshua C. and Dunkle, Jack A.
Journal of Biological Chemistry, vol. 295, pp. 17476–17485. | Journal Article
2019
Direct observation of intermediates in the SufS cysteine desulfurase reaction reveals functional roles of conserved active-site residues
Blahut, Matthew⋅Wise, Courtney E.⋅Bruno, Michael R.⋅Dong, Guangchao⋅Makris, Thomas M.⋅Frantom, Patrick A. and Dunkle, Jack A.
Journal of Biological Chemistry, vol. 294, pp. 12444–12458. | Journal Article
 
Regulation of cyclic oligoadenylate synthesis by the Staphylococcus epidermidis Cas10-Csm complex
Nasef, Mohamed⋅Muffly, Mary C.⋅Beckman, Andrew B.⋅Rowe, Sebastian J.⋅Walker, Forrest C.⋅Hatoum-Aslan, Asma and Dunkle, Jack A.
RNA, vol. 25, pp. 948–962. | Journal Article
2018
Mechanism of tRNA-mediated +1 ribosomal frameshifting.
Hong, Samuel⋅Sunita, S⋅Maehigashi, Tatsuya⋅Hoffer, Eric D⋅Dunkle, Jack A and Dunham, Christine M
Proceedings of the National Academy of Sciences of the United States of America, September 27, 2018. | Journal Article
 
Structural evidence for dimer-interface driven regulation of the type II cysteine desulfurase, SufS.
Dunkle, Jack A⋅Bruno, Michael⋅Outten, Franklin Wayne and Frantom, Patrick A
Biochemistry, December 20, 2018. | Journal Article
2014
Molecular recognition and modification of the 30S ribosome by the aminoglycoside-resistance methyltransferase NpmA
Dunkle, Jack A.⋅Dunkle, Jack A⋅Vinal, Kellie⋅Vinal, Kellie⋅Desai, Pooja M.⋅Desai, Pooja M⋅Zelinskaya, Natalia⋅Zelinskaya, Natalia⋅Savic, Miloje⋅Savic, Miloje⋅West, Dayne M⋅West, Dayne M.⋅Conn, Graeme L⋅Conn, Graeme L.⋅Dunham, Christine M and Dunham, Christine M.
Proceedings of the National Academy of Sciences of the United States of America, vol. 111, (no. 17), Apr 29, 2014. | Journal Article
2012
The mechanisms of RNA SHAPE chemistry.
McGinnis, Jennifer L⋅Dunkle, Jack A⋅Cate, Jamie H D and Weeks, Kevin M
Journal of the American Chemical Society, vol. 134, (no. 15), pp. 6617-6624, April 18, 2012. | Journal Article
2011
Structures of the Bacterial Ribosome in Classical and Hybrid States of tRNA Binding
Dunkle, Jack A⋅Wang, Leyi⋅Feldman, Michael B⋅Pulk, Arto⋅Chen, Vincent B⋅Kapral, Gary J⋅Noeske, Jonas⋅Richardson, Jane S⋅Blanchard, Scott C and Cate, Jamie H
Science, vol. 332, (no. 6032), pp. 981-984, May 20, 2011. | Journal Article
2010
Structures of the Escherichia coli ribosome with antibiotics bound near the peptidyl transferase center explain spectra of drug action.
Dunkle, Jack A⋅Xiong, Liqun⋅Mankin, Alexander S⋅Cate, Jamie H D and Doudna Cate, Jamie H
Proceedings of the National Academy of Sciences of the United States of America, vol. 107, (no. 40), pp. 17152-7, 2010/Oct/5. | Journal Article
2009
Structures of the Ribosome in Intermediate States of Ratcheting
Zhang, Wen⋅Dunkle, Jack A and Cate, Jamie H
Science, vol. 325, (no. 5943), pp. 1014-1017, Aug 21, 2009. | Journal Article