This laboratory uses Baker's yeast to study the regulation of early cell cycle events required for the initiation of S phase and DNA replication. We have found that an enzyme responsible for attaching the highly conserved protein ubiquitin onto other protein substrates is necessary for the transition into S phase. Our immediate goals are to determine how this ubiquitin-conjugating activity is regulated as well as to identify its substrate proteins. A variety of molecular, genetic, and immunochemical approaches will be employed.

Natural Resources Conservation, Biochemistry, Molecular Biology, Cellular Biochemistry, Molecular Biochemistry
PhD, University of Chicago, Molecular Genetics and Cell Biology, 1985
BS, University of Notre Dame, Microbiology, 1980
cancer biology gene cloning molecular cloning molecular probes nucleic acid sequencing cell & developmental biology genetics molecular genetics